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Once plants are generated by tissue culture, they have to be transferred to the greenhouse or field. This requires that the plants be hardened-off before transfer to the field. To reduce water loss during acclimatization, plants are initially transferred to…

The success of acclimatization of a plantlet greatly depends on root system production. Rooting of shoots can be achieved in vitro or ex-vitro. Ex vitro rooting involves pretreating the shoots with phenols or auxins and then planting them directly in…

It includes two methods: Organogenesis  In plant tissue culture, it refers to the formation of either shoot or root. The equilibrium of auxin and cytokinin and the tissue’s capacity to react to phytohormones during culture are key factors in in-vitro…

Callus Culture  A callus is an unorganized mass of cells that develops when cells are wounded. When the explant is cultivated on media that promote the development of undifferentiated cells, a callus is formed. The majority of callus cells are…

The choice of medium is based on the types of plant species; explants are used for culture for optimal response. All the nutrients required for a plant’s proper growth and development should be present in the plant tissue culture media.…

Plant tissue culture is the in-vitro aseptic culture of cells, tissues, or whole plants under controlled nutritional and environmental conditions, often to produce clones of plants. The technique primarily relies on plant cells’ totipotency which is the capacity of a single cell…