Rooting of shoots

The success of acclimatization of a plantlet greatly depends on root system production. Rooting of shoots can be achieved in vitro or ex-vitro.

Ex vitro rooting involves pretreating the shoots with phenols or auxins and then planting them directly in soil under high humidity, which significantly lowers the cost of manufacturing. This technique also allows simultaneous acclimation of the rooted shoots.

In vitro rooting consists of rooting the plants in axenic conditions. Despite the cost factor, in vitro rooting is still a common practice in many plant species.

Several factors are known to affect rooting. The most important factor is the action of endogenous and exogenous auxins. Phenolic compounds are also known to have a stimulatory effect on rooting. Phloroglucinol, a root promoter, is reported effective in root development. Catechol, a strong reducing agent, has been reported to regulate IAA oxidation.