Acclimation / Acclimatization

Once plants are generated by tissue culture, they have to be transferred to the greenhouse or field. This requires that the plants be hardened-off before transfer to the field. To reduce water loss during acclimatization, plants are initially transferred to a greenhouse or growth chamber. The relative humidity outside the vessels is often significantly lower than the humidity inside the vessels. Once the plants are acclimatized under greenhouse conditions, they are ready for transfer to the field.